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Literature summary extracted from

  • Bruender, N.A.; Grell, T.A.; Dowling, D.P.; McCarty, R.M.; Drennan, C.L.; Bandarian, V.
    7-Carboxy-7-deazaguanine synthase a radical S-adenosyl-L-methionine enzyme with polar tendencies (2017), J. Am. Chem. Soc., 139, 1912-1920 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.99.3 X-ray crystal structure of dimeric His6 tagged enzyme is determined to 2.55 A resolution by Multiwavelength Anomalous Dispersion Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.99.3 Mg2+ Mg2+ provides key contacts with the substrate 6-carboxy-5,6,7,8-tetrahydropterin and the product 7-carboxy-7-carbaguanine, thus anchoring the molecules in the active site. The formation of either 6-carboxypterin product is not enhanced by the presence of Mg2+ Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin Bacillus subtilis the enzyme catalyzes a key step in the biosynthesis of 7-deazapurine containing natural products 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin Bacillus subtilis 168 the enzyme catalyzes a key step in the biosynthesis of 7-deazapurine containing natural products 7-carboxy-7-carbaguanine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.99.3 Bacillus subtilis O31677
-
-
4.3.99.3 Bacillus subtilis 168 O31677
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin
-
 Bacillus subtilis 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin the enzyme catalyzes a key step in the biosynthesis of 7-deazapurine containing natural products Bacillus subtilis 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin
-
 Bacillus subtilis 168 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxy-5,6,7,8-tetrahydropterin the enzyme catalyzes a key step in the biosynthesis of 7-deazapurine containing natural products Bacillus subtilis 168 7-carboxy-7-carbaguanine + NH3
-
 ?
4.3.99.3 6-carboxypterin under reducing conditions that would promote the reductive cleavage of S-adenosyl-L-methionine, 6-carboxypterin is turned over to 6-deoxyadenosylpterin, presumably by radical addition of the 5'-deoxyadenosine followed by oxidative decarboxylation to the product. In the absence of the strong reductant, dithionite, the carboxylate of 6-carboxypterin is esterified to generate 6-carboxypterin-5'-deoxyadenosyl ester Bacillus subtilis ?
-
 ?
4.3.99.3 6-carboxypterin under reducing conditions that would promote the reductive cleavage of S-adenosyl-L-methionine, 6-carboxypterin is turned over to 6-deoxyadenosylpterin, presumably by radical addition of the 5'-deoxyadenosine followed by oxidative decarboxylation to the product. In the absence of the strong reductant, dithionite, the carboxylate of 6-carboxypterin is esterified to generate 6-carboxypterin-5'-deoxyadenosyl ester Bacillus subtilis 168 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.99.3 CDG synthase
-
 Bacillus subtilis
4.3.99.3 queE
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.99.3 4Fe-4S-center S-adenosyl-L-methionine binds to the unique iron atom of a site-differentiated [4Fe-4S] cluster and is reductively cleaved to generate a 5'-deoxyadenosyl radical, which initiates turnover Bacillus subtilis
4.3.99.3 S-adenosyl-L-methionine S-adenosyl-L-methionine-dependent radical enzyme Bacillus subtilis

General Information

EC Number General Information Comment Organism
4.3.99.3 metabolism the enzyme catalyzes a key step in the biosynthesis of 7-deazapurine containing natural products Bacillus subtilis